Due to various new aspects (e.g. relevance of disinfectants containing remanence agents) and new clinical focuses (e.g. hygiene aspects of ultrasound-guided procedures, regional anesthesia techniques outside the OR), the previous recommendations (“10 commandments”) have been updated in a new edition under the auspices of the German Society of Anesthesiology and Intensive Care Medicine (DGAI). This update is based on the old recommendations, which on the one hand have remained largely unchanged in terms of content, but on the other hand have been formulated much more clearly in response to frequent requests from specialist colleagues in recent years and have now been published as an S1 guideline (AWMF register number 001-044).